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England flanker Chris Robshaw requires shoulder surgery that will rule him out of the Six Nations.
Harlequins on Monday revealed that the former England captain must undergo an operation that is expected to keep him out for 12 weeks.
The 30-year-old was injured during Quins' Premiership defeat at Worcester Warriors on New Year's Day and will be unavailable to Eddie Jones for England's defence of their Six Nations title.
Robshaw has been outstanding since Jones opted to strip him of the captaincy and appoint Dylan Hartley as skipper.
He played a key role in England's Grand Slam triumph last year and maintained those high standards on the successful tour of Australia.
Robshaw also started when England beat the Wallabies at Twickenham last month to extend their winning run to 14 matches, but he will play no part in the Six Nations.
The loss of Robshaw is another big blow to Jones, who has just a few weeks to decide on the make-up of his back row for the clash against France at Twickenham on February 4.
Barnstorming number eight Billy Vunipola will also miss the tournament due to a knee injury, while George Kruis is a doubt for the clash with Les Bleus due to a fractured cheekbone.
James Haskell lasted just 35 seconds of his Wasps comeback against Leicester Tigers on Sunday after suffering concussion, but was able to walk off the field, while Joe Launchbury is on the mend following a calf problem.
Maro Itoje could move to number six in the absence of Robshaw.
|1||Chris Barnby||186 pts|
|2||Ben Harrison||176 pts|
|3||Andrew Ewing||175 pts|